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Producto Referencia Descripción Formato PDF Detalles
IL-1α (rat) ELISA Kit K4797-100 BioVision’s rat IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-lin... 100 assays N/D
IL-1α (rat) ELISA Kit » Descripción

BioVision’s rat IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 alpha. This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The minimum detectable dose of IL-1 alpha is typically less than 15 pg/ml. Detection Range: 15 pg/ml – 8000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity-rat • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Cell culture supernatants

IL-1β (rat) ELISA Kit K4796-100 BioVision’s rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-link... 100 assays N/D
IL-1β (rat) ELISA Kit » Descripción

BioVision’s rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta. This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 80 pg/ml. Detection Limit: 80 pg/ml – 50000 pg/ml.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity-rat • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Cell culture supernatants

IL-1β (mouse) ELISA Kit K4795-100 BioVision’s mouse IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-li... 100 assays N/D
IL-1β (mouse) ELISA Kit » Descripción

BioVision’s mouse IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta. This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1α, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13,IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 5 pg/ml. Detection Limit: 5 pg/ml – 2000 pg/ml.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity-mouse • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Cell culture supernatants

IL-1β (human) ELISA Kit K4794-100 BioVision’s Human IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-li... 100 assays N/D
IL-1β (human) ELISA Kit » Descripción

BioVision’s Human IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 beta. This assay employs an antibody specific for human IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of IL-1 beta is typically less than 0.3 pg/ml. Detection Range: 0.3 pg/ml – 100 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity-human • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma (human IL-1 beta conc. is pretty low in normal serum/plasma, it may not be detected in this assay) • Cell culture supernatants • Urine

IL-1α (mouse) ELISA Kit K4793-100 BioVision’s mouse IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-l... 100 assays N/D
IL-1α (mouse) ELISA Kit » Descripción

BioVision’s mouse IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 alpha. This assay employs an antibody specific for human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF). The minimum detectable dose of IL-1 alpha is typically less than 10 pg/ml. Detection Range: 10 pg/ml – 3000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity-mouse • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Cell culture supernatants

IL-1α (human) ELISA Kit K4791-100 BioVision’s Human IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-l... 100 assays N/D
IL-1α (human) ELISA Kit » Descripción

BioVision’s Human IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 alpha. This assay employs an antibody specific for human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of IL-1 alpha is typically less than 0.5 pg/ml. Detection Range: 0.5 pg/ml – 300 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Cell culture supernatants • Urine

TNF-beta (human) ELISA Kit K4780-100 BioVision’s Human TNF-beta (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay)... 100 assays N/D
TNF-beta (human) ELISA Kit » Descripción

BioVision’s Human TNF-beta (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-beta. This assay employs an antibody specific for human TNF-beta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL- 12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TPO, VEGF. The minimum detectable dose of TNF-beta is typically less than 60 pg/ml. Detection Range: 60 pg/ml – 18,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma (human TNF-beta concentration is pretty low in normal serum & plasma, it may not be detected in this assay) • Cell culture supernatants • Urine

Ghrelin (human, mouse, rat) EIA Kit K4790-100 BioVision’s Ghrelin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting G... 100 assays N/D
Ghrelin (human, mouse, rat) EIA Kit » Descripción

BioVision’s Ghrelin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Ghrelin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Ghrelin antibody, both biotinylated Ghrelin peptide and peptide standard or targeted peptide in samples interacts competitively with the Ghrelin antibody. Uncompeted (bound) biotinylated Ghrelin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Ghrelin peptide in the standard or samples. This is due to the competitive binding of Ghrelin antibody between biotinylated Ghrelin peptide and peptides in standard or samples. A standard curve of known concentration of Ghrelin peptide can be established and the concentration of Ghrelin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Ghrelin is 161 pg/ml or 12.46 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%. This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Nesfatin, Angiotensin II, NPY and APC.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human, mouse, rat • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Cell culture media or other sample types

TNF-alpha (human) ELISA Kit K4779-100 BioVision’s Human TNF-alpha (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay... 100 assays N/D
TNF-alpha (human) ELISA Kit » Descripción

BioVision’s Human TNF-alpha (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-alpha. This assay employs an antibody specific for human TNF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ1, TGFβ2, TGFβ3, VEGF. The minimum detectable dose of TNF-alpha is typically less than 30 pg/ml. Detection Range: 30 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma (human TNF-alpha concentration is pretty low in normal serum & plasma, it may not be detected in this assay) • Cell culture supernatants • Urine

RELM-Alpha (human, mouse, rat) EIA Kit K4789-100 BioVision’s RELM-alpha (also known as FIZZ1, HIMF) Enzyme Immunoassay (EIA) Kit is an in vitro qua... 100 assays N/D
RELM-Alpha (human, mouse, rat) EIA Kit » Descripción

BioVision’s RELM-alpha (also known as FIZZ1, HIMF) Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting RELM-alpha peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-RELM-alpha antibody. After a blocking step, both biotinylated RELM-alpha peptide and peptide standard or targeted peptide in samples interacts competitively with the RELM-alpha antibody. Uncompeted (bound) biotinylated RELM-alpha peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of RELM-alpha peptide in the standard or samples. This is due to the competitive binding to RELM-alpha antibody between biotinylated RELM-alpha peptide and peptides in standard or samples. A standard curve of known concentration of RELM-alpha peptide can be established and the concentration of RELM-alpha peptide in the samples can be calculated accordingly. The minimum detectable concentration of RELM-alpha is 4.7 ng/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%. This EIA kit detects RELM-alpha (88 aa). No other active isoforms have been reported. This kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, NPY and APC.

» Información

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- human, mouse, rat • Application- Quantitative protein detection, establishing normal range, validation of antibody array results

» Más Datos

Shipping Conditions: gel pack, Storage Conditions: -20°C

» Extras

Sample Type: • Serum & plasma • Culture supernatants & other samples

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